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KMID : 1025520070490020183
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Journal of Animal Science and Technology
2007 Volume.49 No. 2 p.183 ~ p.194
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Screening of Chicken Genes Related to Germ Cell Development
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Lee Jee-Young
Kim Hee-Bal
Kim Duk-Kyung
Song Ki-Duk
Lim Jeong-Mook
Han Jae-Yong
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Abstract
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We examined the expression patterns of the chicken TCs(tentative consensus sequences) originated from GermOnline genes in various chicken tissues, applying information from GermOnline to chicken organisms. 42 TCs among 84 chicken homologous TCs from the pool of 84 genes related to germ cell lineage in mouse(10), rat(71) and human(3) had high homology based on a BLAST search. Of these, Hmgcs2 and Sycp3 was shown to be expressed in a testis- specific manner and a reproductive organ(testis and ovary)-specific manner, respectively, by RT- PCR analysis. Crmp4, Cyct, Ldhc, Epha7, Pcsk4 and Dnmt3a are expressed in brain, testis, and ovary. The characterization of chicken genes originated from GermOnline in this research may give an enormously useful source of information related to germ cell development.
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KEYWORD
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Chicken, Germ cell, GermOnline, Hmgcs2, Sycp3
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